ADRIANA SCARLAT*, C. BÎSCEANU*, C. CĂPITĂNESCU***, I. GH. TANASE***, S. RADU*****, L. CHIRIGIU*****, GABRIELA RĂU*****
* Doctorand, Facultatea de Farmacie, UMF Craiova ** Agentia de Protecţia Medicului, Drobeta Turnu-Severin, Romania *** Disciplina de Chimie analitică, Facultatea de Chimie, Universitatea Bucureşti **** Facultatea de Farmacie, U. M. F. Craiova
SUMMARY:
The detailed analysis of those methods allow us the following commol/Lents: the methods of L-aminoacids determination by assaying H2O2 using peroxidase and N,Ndimethyl- p-phenylendiamine or p-phenylendiamine are simple and sensitive but the solutions of chromogens are not very stable (1 – 2 months in a cold place) and cancerous; the method of assaying H2O2 using peroxidase and 4-aminoantipyrine/phenol is fit for a lab purposes and possibilities taking into account the working steps, the reaction time (2 steps – solution with combined reagents, 15 / 20 minutes/determination), the acceptable toxicity and good stability of reagents.
* Doctorand, Facultatea de Farmacie, UMF Craiova ** Agentia de Protecţia Medicului, Drobeta Turnu-Severin, Romania *** Disciplina de Chimie analitică, Facultatea de Chimie, Universitatea Bucureşti **** Facultatea de Farmacie, U. M. F. Craiova
SUMMARY:
The detailed analysis of those methods allow us the following commol/Lents: the methods of L-aminoacids determination by assaying H2O2 using peroxidase and N,Ndimethyl- p-phenylendiamine or p-phenylendiamine are simple and sensitive but the solutions of chromogens are not very stable (1 – 2 months in a cold place) and cancerous; the method of assaying H2O2 using peroxidase and 4-aminoantipyrine/phenol is fit for a lab purposes and possibilities taking into account the working steps, the reaction time (2 steps – solution with combined reagents, 15 / 20 minutes/determination), the acceptable toxicity and good stability of reagents.